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《医学前沿(英文)》 2013年 第7卷 第4期 页码 486-491 doi: 10.1007/s11684-013-0287-x
The purpose of this study was to compare cell growth characteristics, ciliated cell differentiation, and function of human nasal epithelial cells established as explant outgrowth cultures or dissociated tissue cultures. Human nasal mucosa of the uncinate process was obtained by endoscopy and epithelial cell cultures were established by explant outgrowth or dissociated tissue culture methods. Epithelial cell growth characteristics were observed by inverted phase contrast microscopy. Ciliated cell differentiation was detected by β-tubulin IV and ZO-1 immunocytochemistry. Basal and ATP-stimulated ciliary beat frequency (CBF) was measured using a high-speed digital microscopic imaging system. Both the explant and dissociated tissue cultures established as monolayers with tight junctions and differentiated cell composition, with both types of cultures comprising ciliated and non-ciliated epithelial cells. Fibroblasts were also frequently found in explant cultures but rarely seen in dissociated tissue cultures. In both culture systems, the highest ciliated cell density appeared at 7th–10th culture day and declined with time, with the lifespan of ciliated cells ranging from 14 to 21 days. Overall, 10% of the cells in explant cultures and 20% of the cells in the dissociated tissue cultures were ciliated. These two cultures demonstrated similar ciliary beat frequency values at baseline (7.78±1.99 Hz and 7.91±2.52 Hz, respectively) and reacted equivalently following stimulation with 100 μM ATP. The results of this study indicate that both the explant outgrowth and dissociated tissue culture techniques are suitable for growing well-differentiated nasal ciliated and non-ciliated cells, which have growth characteristics and ciliary activity similar to those of nasal epithelial cells in vivo.
关键词: ciliated cells ciliary beat frequency dissociated tissue culture explant culture nasal epithelial cells
Case report of neurofibromatosis type 1 combined with primary ciliary dyskinesia
《医学前沿(英文)》 2021年 第15卷 第6期 页码 933-937 doi: 10.1007/s11684-021-0860-7
关键词: primary ciliary dyskinesia neurofibromatosis bronchiectasis transmission electron microscopy genetic sequencing
Lack of CFAP54 causes primary ciliary dyskinesia in a mouse model and human patients
《医学前沿(英文)》 doi: 10.1007/s11684-023-0997-7
Dynein axonemal heavy chain 10 deficiency causes primary ciliary dyskinesia in humans and mice
《医学前沿(英文)》 页码 957-971 doi: 10.1007/s11684-023-0988-8
关键词: DNAH10 mice motile cilia mutation primary ciliary dyskinesia
Solid-state transformer-based new traction drive system and control
Jianghua FENG, Jing SHANG, Zhixue ZHANG, Huadong LIU, Zihao HUANG
《机械工程前沿(英文)》 2018年 第13卷 第3期 页码 411-426 doi: 10.1007/s11465-018-0467-0
A new type of traction drive system consisting of solid-state traction transformer (SSTT), inverter unit, auxiliary inverter, traction motor and other key components is built in order to suit the demand of developing the next-generation electric traction system which will be efficient and lightweight, with high power density. For the purpose of reducing system volume and weight and improving efficiency and grid-side power quality, an efficient SSTT optimized topology combining high-voltage cascaded rectifiers with high-power high-frequency LLC resonant converter is proposed. On this basis, an integrated control strategy built upon synchronous rotating reference frame is presented to achieve unified control over fundamental active, reactive and harmonic components. The carrier-interleaving phase shift modulation strategy is proposed to improve the harmonic performance of cascaded rectifiers. In view of the secondary pulsating existing in a single-phase system, the mathematical model of secondary power transfer is built, and the mechanism of pulsating voltage resulting in beat frequency of LLC resonant converter is revealed, so as to design optimum matching of system parameters. Simulation and experimental results have verified that the traction system and control scheme mentioned in this paper are reasonable and superior and that they meet the future application requirements for rail transit.
关键词: solid-state traction transformer high-voltage cascaded rectifier LLC resonant converter synchronous rotating reference frame carrier-interleaving phase shift control secondary pulsating voltage beat frequency
用于并网逆变器的改进三矢量无差拍模型预测直接功率控制策略 None
Chen-wen CHENG, Heng NIAN, Long-qi LI
《信息与电子工程前沿(英文)》 2018年 第19卷 第11期 页码 1420-1431 doi: 10.1631/FITEE.1601874
A frequency error estimation for isogeometric analysis of Kirchhoff–Love cylindrical shells
《结构与土木工程前沿(英文)》 doi: 10.1007/s11709-023-0006-x
关键词: isogeometric analysis Kirchhoff–Love cylindrical shell free vibration frequency error convergence
Xiaowei LUO, Yannick BENICHOU, Suyuan YU
《能源前沿(英文)》 2013年 第7卷 第4期 页码 506-510 doi: 10.1007/s11708-013-0275-5
关键词: aerosol particles collision frequency function coagulation
Zhigen WU, Guohua LIU, Zihua ZHANG
《结构与土木工程前沿(英文)》 2011年 第5卷 第1期 页码 112-120 doi: 10.1007/s11709-010-0069-3
关键词: damage assessment decay ratio of acceleration signals (DRAS) wavelet transform (WT) modal analysis reinforced concrete beam beat phenomenon
Fuel variability effect on flickering frequency of diffusion flames
Jizhao LI, Yang ZHANG
《能源前沿(英文)》 2009年 第3卷 第2期 页码 134-140 doi: 10.1007/s11708-009-0034-9
关键词: fuel variability flickering frequency diffusion flames dynamics of combustion
Capacity limitation of nuclear units in grid based on analysis of frequency regulation
Yiping DAI, Pan JIANG, Lin GAO, Weimin KAN, Xiaoqing XIAO, Ge JIN
《能源前沿(英文)》 2012年 第6卷 第2期 页码 148-154 doi: 10.1007/s11708-012-0174-1
关键词: nuclear units capacity overspeed primary frequency control (PFC) second frequency control (SFC)
Oscillation frequency of simplified arterial tubes
Hui AN, Fan HE, Lingxia XING, Xiaoyang LI
《机械工程前沿(英文)》 2009年 第4卷 第3期 页码 300-304 doi: 10.1007/s11465-009-0038-5
关键词: spatial kinematics natural frequency finite element method
Xuzhan ZHOU,Shanshan LIU,Mingkun WANG,Yiping DAI,Yaohua TANG
《能源前沿(英文)》 2014年 第8卷 第3期 页码 386-393 doi: 10.1007/s11708-014-0323-9
关键词: high voltage (HV) grid frequency stability parameter identification primary frequency regulation (PFR)
Dependence of error sensitivity of frequency on bias voltage in force-balanced micro accelerometer
Lili CHEN, Wu ZHOU
《机械工程前沿(英文)》 2013年 第8卷 第2期 页码 146-149 doi: 10.1007/s11465-013-0260-z
To predict more precisely the frequency of force-balanced micro accelerometer with different bias voltages, the effects of bias voltages on error sensitivity of frequency is studied. The resonance frequency of accelerometer under closed loop control is derived according to its operation principle, and its error sensitivity is derived and analyzed under over etching structure according to the characteristics of Deep Reaction Ion Etching (DRIE). Based on the theoretical results, micro accelerometer is fabricated and tested to study the influences of AC bias voltage and DC bias voltage on sensitivity, respectively. Experimental results indicate that the relative errors between test data and theory data are less than 7%, and the fluctuating value of error sensitivity under the range of voltage adjustment is less than 0.01 μm . It is concluded that the error sensitivity with designed parameters of structure, circuit and process error can be used to predict the frequency of accelerometer with no need to consider the influence of bias voltage.
关键词: Micro-Electro-Mechanical Systems (MEMS) micro accelerometer force-balanced micro accelerometer frequency error sensitivity
《医学前沿(英文)》 2023年 第17卷 第3期 页码 493-502 doi: 10.1007/s11684-022-0946-x
关键词: ALK fusion next-generation sequencing fluorescence in situ hybridization immunohistochemistry variant allele frequency intratumoral heterogeneity targeted therapy
标题 作者 时间 类型 操作
Comparison of human nasal epithelial cells grown as explant outgrowth cultures or dissociated tissue cultures
null
期刊论文
Solid-state transformer-based new traction drive system and control
Jianghua FENG, Jing SHANG, Zhixue ZHANG, Huadong LIU, Zihao HUANG
期刊论文
Calculation of collision frequency function for aerosol particles in free molecule regime in presence
Xiaowei LUO, Yannick BENICHOU, Suyuan YU
期刊论文
Experimental study of structural damage identification based on modal parameters and decay ratio of acceleration signals
Zhigen WU, Guohua LIU, Zihua ZHANG
期刊论文
Capacity limitation of nuclear units in grid based on analysis of frequency regulation
Yiping DAI, Pan JIANG, Lin GAO, Weimin KAN, Xiaoqing XIAO, Ge JIN
期刊论文
Parameter identification of interconnected power system frequency after trip-out of high voltage transmission
Xuzhan ZHOU,Shanshan LIU,Mingkun WANG,Yiping DAI,Yaohua TANG
期刊论文
Dependence of error sensitivity of frequency on bias voltage in force-balanced micro accelerometer
Lili CHEN, Wu ZHOU
期刊论文